› 4mhq

X-ray diffraction
2.2Å resolution

Crystal structure of human primase catalytic subunit

Released:
DOI: 10.2210/pdb4mhq/pdb
PDB entry 4mhq coloured by chain, front view.
PDB entry 4mhq coloured by chain, side view.
PDB entry 4mhq coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Park KR, An JY, Lee Y, Youn HS, Lee JG, Kang JY, Kim TG, Lim JJ, Eom SH, Wang J

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase small subunit Chain: A
Molecule details ›
Chain: A
Length: 425 amino acids
Theoretical weight: 50.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49642 (Residues: 1-420; Coverage: 100%)
Gene name: PRIM1
Sequence domains: DNA primase small subunit
Structure domains: DNA primase, PRIM domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P43212
Unit cell:
a: 84.762Å b: 84.762Å c: 147.622Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.233
Expression system: Escherichia coli

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