› 4mhx

X-ray diffraction
2Å resolution

Crystal Structure of Sulfamidase

Released:
DOI: 10.2210/pdb4mhx/pdb
PDB entry 4mhx coloured by chain, front view.
PDB entry 4mhx coloured by chain, side view.
PDB entry 4mhx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.
Sidhu NS, Schreiber K, Pröpper K, Becker S, Usón I, Sheldrick GM, Gärtner J, Krätzner R, Steinfeld R
Acta Crystallogr D Biol Crystallogr 70 1321-35 (2014)
PMID: 24816101

Function and Biology Details

Reaction catalysed: 
N-sulfo-D-glucosamine + H(2)O = D-glucosamine + sulfate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
N-sulphoglucosamine sulphohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 510 amino acids
Theoretical weight: 57.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51688 (Residues: 1-502; Coverage: 100%)
Gene names: HSS, SGSH
Sequence domains:
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand NAG 4 x NAG
Ligand MG 2 x MG
1 modified residue:
Ligand FGP 2 x FGP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 61.38Å b: 107.9Å c: 79.79Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.194 0.192 0.23
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Formylglycine, a post-translationally generated residue with unique catalytic capabilities and biotechnology applications.
Appel et al. (2015)
12 other articles

5 mentions without citation

Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.
Sidhu et al. (2014)
4 more