› 4miv

X-ray diffraction
2.4Å resolution

Crystal Structure of Sulfamidase, Crystal Form L

Released:
DOI: 10.2210/pdb4miv/pdb
PDB entry 4miv coloured by chain, front view.
PDB entry 4miv coloured by chain, side view.
PDB entry 4miv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.
Sidhu NS, Schreiber K, Pröpper K, Becker S, Usón I, Sheldrick GM, Gärtner J, Krätzner R, Steinfeld R
Acta Crystallogr D Biol Crystallogr 70 1321-35 (2014)
PMID: 24816101

Function and Biology Details

Reaction catalysed: 
N-sulfo-D-glucosamine + H(2)O = D-glucosamine + sulfate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
N-sulphoglucosamine sulphohydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 510 amino acids
Theoretical weight: 57.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51688 (Residues: 1-502; Coverage: 100%)
Gene names: HSS, SGSH
Sequence domains:
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand CA 8 x CA
Ligand NAG 9 x NAG
Ligand CL 2 x CL
Ligand PEG 1 x PEG
1 modified residue:
Ligand FGP 8 x FGP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 102.93Å b: 211.45Å c: 108.35Å
α: 90° β: 102.69° γ: 90°
R-values:
R R work R free
0.217 0.216 0.245
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Formylglycine, a post-translationally generated residue with unique catalytic capabilities and biotechnology applications.
Appel et al. (2015)
13 other articles

4 mentions without citation

Insights into the Mechanistic Basis of Plasmid-Mediated Colistin Resistance from Crystal Structures of the Catalytic Domain of MCR-1.
Hinchliffe et al. (2017)
3 more