› 4mj4

X-ray diffraction
2.17Å resolution

Human iduronidase apo structure P21 form

Released:
DOI: 10.2210/pdb4mj4/pdb
PDB entry 4mj4 coloured by chain, front view.
PDB entry 4mj4 coloured by chain, side view.
PDB entry 4mj4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into mucopolysaccharidosis I from the structure and action of α-L-iduronidase.
Bie H, Yin J, He X, Kermode AR, Goddard-Borger ED, Withers SG, James MN
Nat Chem Biol 9 739-45 (2013)
PMID: 24036510

Function and Biology Details

Reaction catalysed: 
Hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-L-iduronidase Chain: A
Molecule details ›
Chain: A
Length: 653 amino acids
Theoretical weight: 72.69 KDa
Source organism: Homo sapiens
Expression system: Arabidopsis thaliana
UniProt:
  • Canonical: P35475 (Residues: 1-653; Coverage: 100%)
Gene name: IDUA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand GOL 3 x GOL
Ligand NAG 2 x NAG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 63.335Å b: 70.507Å c: 67.303Å
α: 90° β: 92.1° γ: 90°
R-values:
R R work R free
0.203 0.201 0.24
Expression system: Arabidopsis thaliana

Quick links

Citations

8 review citations

Dissecting conformational contributions to glycosidase catalysis and inhibition.
Speciale et al. (2014)
7 more

1 mention without citation

Insights into mucopolysaccharidosis I from the structure and action of α-L-iduronidase.
Bie et al. (2013)