› 4mjh

X-ray diffraction
2.6Å resolution

Human Hsp27 core domain in complex with C-terminal peptide

Released:
DOI: 10.2210/pdb4mjh/pdb
PDB entry 4mjh coloured by chain, front view.
PDB entry 4mjh coloured by chain, side view.
PDB entry 4mjh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structured core domain of αB-crystallin can prevent amyloid fibrillation and associated toxicity.
Hochberg GK, Ecroyd H, Liu C, Cox D, Cascio D, Sawaya MR, Collier MP, Stroud J, Carver JA, Baldwin AJ, Robinson CV, Eisenberg DS, Benesch JL, Laganowsky A
Proc Natl Acad Sci U S A 111 E1562-70 (2014)
PMID: 24711386

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138179 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock protein beta-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 93 amino acids
Theoretical weight: 10.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04792 (Residues: 84-176; Coverage: 45%)
Gene names: HSP27, HSP28, HSPB1
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like
Heat shock protein beta-1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 8 amino acids
Theoretical weight: 919 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04792 (Residues: 179-186; Coverage: 4%)
Gene names: HSP27, HSP28, HSPB1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P21
Unit cell:
a: 31.28Å b: 48.96Å c: 57.54Å
α: 90° β: 99.95° γ: 90°
R-values:
R R work R free
0.236 0.234 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

27 review citations

A first line of stress defense: small heat shock proteins and their function in protein homeostasis.
Haslbeck et al. (2015)
26 more

22 mentions without citation

Protein-Protein Interactions in the Molecular Chaperone Network.
Freilich et al. (2018)
21 more