4mji

X-ray diffraction
2.99Å resolution

T cell response to a HIV reverse transcriptase epitope presented by the protective allele HLA-B*51:01

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 276 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
HIV Reverse Transcriptase peptide Marker Chains: C, H
Molecule details ›
Chains: C, H
Length: 8 amino acids
Theoretical weight: 849 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: R4WL38 (Residues: 227-234; Coverage: 2%)
Gene name: pol
Ig-like domain-containing protein Chains: D, I
Molecule details ›
Chains: D, I
Length: 195 amino acids
Theoretical weight: 21.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: K7N5M3 (Residues: 7-203; Coverage: 89%)
Gene names: B2M, HDCMA22P
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: E, J
Molecule details ›
Chains: E, J
Length: 242 amino acids
Theoretical weight: 26.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 45.51Å b: 88.87Å c: 129.27Å
α: 102.91° β: 95.92° γ: 90.09°
R-values:
R R work R free
0.245 0.242 0.304
Expression systems:
  • Escherichia coli
  • Not provided