› 4mov

X-ray diffraction
1.45Å resolution

1.45 A Resolution Crystal Structure of Protein Phosphatase 1

Released:
DOI: 10.2210/pdb4mov/pdb
PDB entry 4mov coloured by chain, front view.
PDB entry 4mov coloured by chain, side view.
PDB entry 4mov coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Understanding the antagonism of retinoblastoma protein dephosphorylation by PNUTS provides insights into the PP1 regulatory code.
Choy MS, Hieke M, Kumar GS, Lewis GR, Gonzalez-DeWhitt KR, Kessler RP, Stein BJ, Hessenberger M, Nairn AC, Peti W, Page R
Proc Natl Acad Sci U S A 111 4097-102 (2014)
PMID: 24591642

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 34.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 7-300; Coverage: 89%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand PO4 2 x PO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 65.724Å b: 77.6Å c: 133.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.166
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Toxin-antitoxin systems in bacterial growth arrest and persistence.
Page et al. (2016)
10 more

23 mentions without citation

Phosphatases in Mitosis: Roles and Regulation.
Moura et al. (2019)
22 more