› 4mqs

X-ray diffraction
3.5Å resolution

Structure of active human M2 muscarinic acetylcholine receptor bound to the agonist iperoxo

Released:
DOI: 10.2210/pdb4mqs/pdb
PDB entry 4mqs coloured by chain, front view.
PDB entry 4mqs coloured by chain, side view.
PDB entry 4mqs coloured by chain, top view.
Source organisms:
Primary publication:
Activation and allosteric modulation of a muscarinic acetylcholine receptor.
Kruse AC, Ring AM, Manglik A, Hu J, Hu K, Eitel K, Hübner H, Pardon E, Valant C, Sexton PM, Christopoulos A, Felder CC, Gmeiner P, Steyaert J, Weis WI, Garcia KC, Wess J, Kobilka BK
Nature 504 101-6 (2013)
PMID: 24256733

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209339 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Muscarinic acetylcholine receptor M2 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08172 (Residues: 1-18, 19-466; Coverage: 70%)
Gene name: CHRM2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins
Nanobody 9-8 Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 13.43 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand IXO 1 x IXO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 62.896Å b: 78.13Å c: 163.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.249 0.298
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

124 review citations

DREADDs for Neuroscientists.
Roth BL. (2016)
123 more

95 mentions without citation

Structure and function of serotonin G protein-coupled receptors.
McCorvy et al. (2015)
94 more