› 4mwf

X-ray diffraction
2.64Å resolution

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core bound to broadly neutralizing antibody AR3C

Released:
DOI: 10.2210/pdb4mwf/pdb
PDB entry 4mwf coloured by chain, front view.
PDB entry 4mwf coloured by chain, side view.
PDB entry 4mwf coloured by chain, top view.
Source organisms:
Primary publication:
Hepatitis C virus E2 envelope glycoprotein core structure.
Kong L, Giang E, Nieusma T, Kadam RU, Cogburn KE, Hua Y, Dai X, Stanfield RL, Burton DR, Ward AB, Wilson IA, Law M
Science 342 1090-4 (2013)
PMID: 24288331

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210943 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Fab AR3C heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 233 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab AR3C light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 214 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Envelope glycoprotein E2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 212 amino acids
Theoretical weight: 23.29 KDa
Source organism: Hepatitis C virus (isolate H)
Expression system: Homo sapiens
UniProt:
  • Canonical: P27958 (Residues: 412-459, 486-645; Coverage: 7%)
Sequence domains: Hepatitis C virus non-structural protein E2/NS1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 47.132Å b: 166.553Å c: 209.963Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.27
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

59 review citations

Immune responses to HCV and other hepatitis viruses.
Park et al. (2014)
58 more

82 mentions without citation

The past, present and future of neutralizing antibodies for hepatitis C virus.
Ball et al. (2014)
81 more