› 4n0c

X-ray diffraction
2.9Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-241027 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, L-D alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 180 amino acids
Theoretical weight: 21.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01897 (Residues: 25-203; Coverage: 53%)
Gene name: H2-L
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Structure domains: MHC class I-like antigen recognition-like
pCPE3 Chains: B, F
Molecule details ›
Chains: B, F
Length: 9 amino acids
Theoretical weight: 1.04 KDa
T cell receptor alpha chain constant; T cell receptor alpha joining 58; T-cell receptor alpha chain V region PHDS58 Chains: C, G
Molecule details ›
Chains: C, G
Length: 212 amino acids
Theoretical weight: 23.38 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P01738 (Residues: 19-113; Coverage: 85%)
  • Canonical: A0A0G2JFA3 (Residues: 3-21; Coverage: 91%)
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC, Traj58
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta joining 2-2 Chains: D, H
Molecule details ›
Chains: D, H
Length: 243 amino acids
Theoretical weight: 27.25 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0A6YX08 (Residues: 4-17; Coverage: 82%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2, Trbj2-2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3121
Unit cell:
a: 102.45Å b: 102.45Å c: 325.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.247
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The evolving landscape of biomarkers for checkpoint inhibitor immunotherapy.
Havel et al. (2019)
13 more

2 mentions without citation

Structural interplay between germline interactions and adaptive recognition determines the bandwidth of TCR-peptide-MHC cross-reactivity.
Adams et al. (2016)
1 more