› 4n0e

X-ray diffraction
2.1Å resolution

Crystal structure of the K345L variant of the Gi alpha1 subunit bound to GDP

Released:
DOI: 10.2210/pdb4n0e/pdb
PDB entry 4n0e coloured by chain, front view.
PDB entry 4n0e coloured by chain, side view.
PDB entry 4n0e coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gαi1.
Thaker TM, Sarwar M, Preininger AM, Hamm HE, Iverson TM
J Biol Chem 289 11331-11341 (2014)
PMID: 24596087

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10824 (Residues: 1-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I4
Unit cell:
a: 121.527Å b: 121.527Å c: 68.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Every Detail Matters. That Is, How the Interaction between Gα Proteins and Membrane Affects Their Function.
Polit et al. (2021)
5 other articles

1 mention without citation

A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gαi1.
Thaker et al. (2014)