› 4n14

X-ray diffraction
2.1Å resolution

Crystal structure of Cdc20 and apcin complex

Released:
DOI: 10.2210/pdb4n14/pdb
PDB entry 4n14 coloured by chain, front view.
PDB entry 4n14 coloured by chain, side view.
PDB entry 4n14 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C.
Sackton KL, Dimova N, Zeng X, Tian W, Zhang M, Sackton TB, Meaders J, Pfaff KL, Sigoillot F, Yu H, Luo X, King RW
Nature 514 646-9 (2014)
PMID: 25156254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division cycle protein 20 homolog Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 34.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q12834 (Residues: 165-477; Coverage: 63%)
Gene name: CDC20
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand WR7 1 x WR7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 41.041Å b: 87.177Å c: 48.043Å
α: 90° β: 112.72° γ: 90°
R-values:
R R work R free
0.168 0.165 0.213
Expression system: Spodoptera frugiperda

Quick links

Citations

48 review citations

Ubiquitin ligases in oncogenic transformation and cancer therapy.
Senft et al. (2018)
47 more

11 mentions without citation

Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C.
Sackton et al. (2014)
10 more