› 4n4w

X-ray diffraction
2.8Å resolution

Structure of the human smoothened receptor in complex with SANT-1.

Released:
DOI: 10.2210/pdb4n4w/pdb
PDB entry 4n4w coloured by chain, front view.
PDB entry 4n4w coloured by chain, side view.
PDB entry 4n4w coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for Smoothened receptor modulation and chemoresistance to anticancer drugs.
Wang C, Wu H, Evron T, Vardy E, Han GW, Huang XP, Hufeisen SJ, Mangano TJ, Urban DJ, Katritch V, Cherezov V, Caron MG, Roth BL, Stevens RC
Nat Commun 5 4355 (2014)
PMID: 25008467

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Protein smoothened; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 53.12 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q0SXH8 (Residues: 23-128; Coverage: 100%)
  • Canonical: Q99835 (Residues: 190-555; Coverage: 48%)
Gene names: SFV_4255, SMO, SMOH, cybC
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand SNT 1 x SNT
Ligand ZN 2 x ZN
Ligand OLC 2 x OLC
Ligand PEG 1 x PEG
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 84.64Å b: 110.88Å c: 145.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.253
Expression system: Spodoptera frugiperda

Quick links

Citations

49 review citations

Hedgehog Signaling: From Basic Biology to Cancer Therapy.
Wu et al. (2017)
48 more

29 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
28 more