Function and Biology Details
Reaction catalysed:
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Cystatin-M and Legumain (preferred)
PDBe Complex ID:
PDB-CPX-172421 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Legumain
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Leishmania tarentolae
UniProt:
Sequence domains: Peptidase C13 family
Structure domains: Rossmann fold
Length: 278 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Leishmania tarentolae
UniProt:
- Canonical:
Q99538 (Residues: 26-303; Coverage: 67%)
Sequence domains: Peptidase C13 family
Structure domains: Rossmann fold
Cystatin-M
Molecule details ›
Chain: B
Length: 131 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
Length: 131 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: Q15828 (Residues: 29-149; Coverage: 100%)
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
Ligands and Environments
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-4
Spacegroup:
P21
Expression systems:
- Leishmania tarentolae
- Escherichia coli
