› 4n7h

X-ray diffraction
1.7Å resolution

Crystal Structure of the Complex of 3rd WW domain of Human Nedd4 and 1st PPXY Motif of ARRDC3

Released:
DOI: 10.2210/pdb4n7h/pdb
PDB entry 4n7h coloured by chain, front view.
PDB entry 4n7h coloured by chain, side view.
PDB entry 4n7h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical basis for ubiquitin ligase recruitment by arrestin-related domain-containing protein-3 (ARRDC3).
Qi S, O'Hayre M, Gutkind JS, Hurley JH
J Biol Chem 289 4743-52 (2014)
PMID: 24379409

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155549 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4 Chain: A
Molecule details ›
Chain: A
Length: 37 amino acids
Theoretical weight: 4.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46934 (Residues: 840-872; Coverage: 3%)
Gene names: KIAA0093, NEDD4, NEDD4-1, PIG53, RPF1
Sequence domains: WW domain
Arrestin domain-containing protein 3 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96B67 (Residues: 342-354; Coverage: 3%)
Gene names: ARRDC3, KIAA1376

Ligands and Environments

1 bound ligand:
Ligand GAI 2 x GAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3221
Unit cell:
a: 38.882Å b: 38.882Å c: 49.997Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Versatile roles of k63-linked ubiquitin chains in trafficking.
Erpapazoglou et al. (2014)
6 more

12 mentions without citation

Exploration of Aberrant E3 Ligases Implicated in Alzheimer's Disease and Development of Chemical Tools to Modulate Their Function.
Potjewyd et al. (2021)
11 more