› 4n7v

X-ray diffraction
2.76Å resolution

Crystal structure of human Plk4 cryptic polo box (CPB) in complex with a Cep152 N-terminal fragment

Released:
DOI: 10.2210/pdb4n7v/pdb
PDB entry 4n7v coloured by chain, front view.
PDB entry 4n7v coloured by chain, side view.
PDB entry 4n7v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis.
Park SY, Park JE, Kim TS, Kim JH, Kwak MJ, Ku B, Tian L, Murugan RN, Ahn M, Komiya S, Hojo H, Kim NH, Kim BY, Bang JK, Erikson RL, Lee KW, Kim SJ, Oh BH, Yang W, Lee KS
Nat Struct Mol Biol 21 696-703 (2014)
PMID: 24997597

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126287 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00444 (Residues: 580-808; Coverage: 24%)
Gene names: PLK4, SAK, STK18
Sequence domains:
Structure domains:
Centrosomal protein of 152 kDa Chain: C
Molecule details ›
Chain: C
Length: 60 amino acids
Theoretical weight: 6.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94986 (Residues: 1-60; Coverage: 4%)
Gene names: CEP152, KIAA0912

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 126.551Å b: 63.942Å c: 79.499Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.258 0.288
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
21 more

2 mentions without citation

Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis.
Park et al. (2014)
1 more