› 4n9j

X-ray diffraction
2.6Å resolution

Crystal structure of the cryptic polo box domain of human Plk4

Released:
DOI: 10.2210/pdb4n9j/pdb
PDB entry 4n9j coloured by chain, front view.
PDB entry 4n9j coloured by chain, side view.
PDB entry 4n9j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis.
Park SY, Park JE, Kim TS, Kim JH, Kwak MJ, Ku B, Tian L, Murugan RN, Ahn M, Komiya S, Hojo H, Kim NH, Kim BY, Bang JK, Erikson RL, Lee KW, Kim SJ, Oh BH, Yang W, Lee KS
Nat Struct Mol Biol 21 696-703 (2014)
PMID: 24997597

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 26.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00444 (Residues: 581-808; Coverage: 24%)
Gene names: PLK4, SAK, STK18
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 6 x CL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P6122
Unit cell:
a: 128.063Å b: 128.063Å c: 168.357Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.202 0.249
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
21 more

3 mentions without citation

STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.
Arquint et al. (2015)
2 more