› 4nc3

X-ray diffraction
2.8Å resolution

Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.

Released:
DOI: 10.2210/pdb4nc3/pdb
PDB entry 4nc3 coloured by chain, front view.
PDB entry 4nc3 coloured by chain, side view.
PDB entry 4nc3 coloured by chain, top view.
Source organisms:
Primary publication:
Serial femtosecond crystallography of G protein-coupled receptors.
Liu W, Wacker D, Gati C, Han GW, James D, Wang D, Nelson G, Weierstall U, Katritch V, Barty A, Zatsepin NA, Li D, Messerschmidt M, Boutet S, Williams GJ, Koglin JE, Seibert MM, Wang C, Shah ST, Basu S, Fromme R, Kupitz C, Rendek KN, Grotjohann I, Fromme P, Kirian RA, Beyerlein KR, White TA, Chapman HN, Caffrey M, Spence JC, Stevens RC, Cherezov V
Science 342 1521-4 (2013)
PMID: 24357322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142091 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxytryptamine receptor 2B; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 430 amino acids
Theoretical weight: 48.28 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41595 (Residues: 36-248, 314-405; Coverage: 63%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: HTR2B, cybC
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand PLM 1 x PLM
Ligand ERM 1 x ERM
Ligand CLR 1 x CLR
Ligand OLC 4 x OLC
Ligand PEG 1 x PEG
Ligand TRS 1 x TRS
Ligand OLA 3 x OLA
Ligand DGA 2 x DGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2221
Unit cell:
a: 61.5Å b: 122.2Å c: 168.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.27
Expression system: Spodoptera frugiperda

Quick links

Citations

50 review citations

Beyond crystallography: diffractive imaging using coherent x-ray light sources.
Miao et al. (2015)
49 more

34 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
33 more