› 4nct

X-ray diffraction
2.6Å resolution

Human DYRK1A in complex with PKC412

Released:
DOI: 10.2210/pdb4nct/pdb
PDB entry 4nct coloured by chain, front view.
PDB entry 4nct coloured by chain, side view.
PDB entry 4nct coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of a dual-specificity tyrosine phosphorylation-regulated kinase 1A-PKC412 complex reveals disulfide-bridge formation with the anomalous catalytic loop HRD(HCD) cysteine.
Alexeeva M, Åberg E, Engh RA, Rothweiler U
Acta Crystallogr D Biol Crystallogr 71 1207-15 (2015)
PMID: 25945585

Function and Biology Details

Reactions catalysed: 
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171790 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity tyrosine-phosphorylation-regulated kinase 1A Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 368 amino acids
Theoretical weight: 42.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13627 (Residues: 126-490; Coverage: 48%)
Gene names: DYRK, DYRK1A, MNB, MNBH
Sequence domains: Protein kinase domain
Structure domains:
Dual specificity tyrosine-phosphorylation-regulated kinase 1A Chain: C
Molecule details ›
Chain: C
Length: 368 amino acids
Theoretical weight: 42.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13627 (Residues: 126-490; Coverage: 48%)
Gene names: DYRK, DYRK1A, MNB, MNBH
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 2K2 4 x 2K2
2 modified residues:
Ligand PTR 4 x PTR
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 87.764Å b: 87.895Å c: 229.921Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.249
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Dyrk1a from Gene Function in Development and Physiology to Dosage Correction across Life Span in Down Syndrome.
Atas-Ozcan et al. (2021)
3 other articles

10 mentions without citation

Dual-Specificity, Tyrosine Phosphorylation-Regulated Kinases (DYRKs) and cdc2-Like Kinases (CLKs) in Human Disease, an Overview.
Lindberg et al. (2021)
9 more