› 4ndy

X-ray diffraction
7Å resolution

Human MHF1-MHF2 DNA complex

Released:
DOI: 10.2210/pdb4ndy/pdb
PDB entry 4ndy coloured by chain, front view.
PDB entry 4ndy coloured by chain, side view.
PDB entry 4ndy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes.
Zhao Q, Saro D, Sachpatzidis A, Singh TR, Schlingman D, Zheng XF, Mack A, Tsai MS, Mochrie S, Regan L, Meetei AR, Sung P, Xiong Y
Nat Commun 5 2987 (2014)
PMID: 24390579

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero octamer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-108751 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Centromere protein S Chains: A, C, G, I, J, K, Q, R, S, T
Molecule details ›
Chains: A, C, G, I, J, K, Q, R, S, T
Length: 105 amino acids
Theoretical weight: 12.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N2Z9 (Residues: 14-105; Coverage: 67%)
Gene names: APITD1, CENPS, FAAP16, MHF1
Sequence domains: CENP-S protein
Centromere protein X Chains: B, D, H, L, M, N, U, V, W, X
Molecule details ›
Chains: B, D, H, L, M, N, U, V, W, X
Length: 74 amino acids
Theoretical weight: 8.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A8MT69 (Residues: 8-81; Coverage: 91%)
Gene names: CENPX, FAAP10, MHF2, STRA13
Sequence domains: CENP-S associating Centromere protein X
DNA (26-MER) Chains: E, O
Molecule details ›
Chains: E, O
Length: 26 nucleotides
Theoretical weight: 8.1 KDa
DNA (26-MER) Chains: F, P
Molecule details ›
Chains: F, P
Length: 26 nucleotides
Theoretical weight: 7.86 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3
Unit cell:
a: 250.098Å b: 250.098Å c: 65.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.279
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Replication fork reversal in eukaryotes: from dead end to dynamic response.
Neelsen et al. (2015)
8 more

1 mention without citation

The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes.
Zhao et al. (2014)