4ne3

X-ray diffraction
1.8Å resolution

Human MHF1-MHF2 complex

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-108754 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centromere protein S Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8N2Z9 (Residues: 14-105; Coverage: 67%)
Gene names: APITD1, CENPS, FAAP16, MHF1
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Centromere protein X Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A8MT69 (Residues: 8-81; Coverage: 91%)
Gene names: CENPX, FAAP10, MHF2, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 61.969Å b: 52.945Å c: 60.283Å
α: 90° β: 114.91° γ: 90°
R-values:
R R work R free
0.232 0.231 0.252
Expression system: Escherichia coli BL21