› 4nfa

X-ray diffraction
2.5Å resolution

Structure of the C-terminal doamin of Knl1

Released:
DOI: 10.2210/pdb4nfa/pdb
PDB entry 4nfa coloured by chain, front view.
PDB entry 4nfa coloured by chain, side view.
PDB entry 4nfa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization.
Petrovic A, Mosalaganti S, Keller J, Mattiuzzo M, Overlack K, Krenn V, De Antoni A, Wohlgemuth S, Cecatiello V, Pasqualato S, Raunser S, Musacchio A
Mol Cell 53 591-605 (2014)
PMID: 24530301

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinetochore scaffold 1 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NG31 (Residues: 2131-2337; Coverage: 9%)
Gene names: CASC5, KIAA1570, KNL1
Sequence domains: Knl1 RWD C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 74.855Å b: 74.855Å c: 88.756Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.19 0.242
Expression system: Escherichia coli

Quick links

Citations

20 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
19 more

1 mention without citation

Inter- vs. Intra-Molecular Hydrogen Bond in Complexes of Nitrophthalic Acids with Pyridine.
Jóźwiak et al. (2023)