› 4nho

X-ray diffraction
2Å resolution

Structure of the spliceosomal DEAD-box protein Prp28

Released:
DOI: 10.2210/pdb4nho/pdb
PDB entry 4nho coloured by chain, front view.
PDB entry 4nho coloured by chain, side view.
PDB entry 4nho coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28.
Möhlmann S, Mathew R, Neumann P, Schmitt A, Lührmann R, Ficner R
Acta Crystallogr D Biol Crystallogr 70 1622-30 (2014)
PMID: 24914973

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent RNA helicase DDX23 Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 55.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BUQ8 (Residues: 338-820; Coverage: 59%)
Gene name: DDX23
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 2 x GOL
Ligand CXS 1 x CXS
Ligand HG 1 x HG
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 125.43Å b: 136.77Å c: 73.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The conformational plasticity of eukaryotic RNA-dependent ATPases.
Ozgur et al. (2015)
3 more

6 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
5 more