› 4nib

X-ray diffraction
1.4Å resolution

Crystal structure of human insulin mutant B20 D-ala, B23 D-ala

Released:
DOI: 10.2210/pdb4nib/pdb
PDB entry 4nib coloured by chain, front view.
PDB entry 4nib coloured by chain, side view.
PDB entry 4nib coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protective hinge in insulin opens to enable its receptor engagement.
Menting JG, Yang Y, Chan SJ, Phillips NB, Smith BJ, Whittaker J, Wickramasinghe NP, Whittaker LJ, Pandyarajan V, Wan ZL, Yadav SP, Carroll JM, Strokes N, Roberts CT, Ismail-Beigi F, Milewski W, Steiner DF, Chauhan VS, Ward CW, Weiss MA, Lawrence MC
Proc Natl Acad Sci U S A 111 E3395-404 (2014)
PMID: 25092300

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand DAL 2 x DAL

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: I213
Unit cell:
a: 78.03Å b: 78.03Å c: 78.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.163
Expression system: Not provided

Quick links

Citations

17 review citations

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
16 more

2 mentions without citation

Protective hinge in insulin opens to enable its receptor engagement.
Menting et al. (2014)
1 more