› 4nqu

X-ray diffraction
2.5Å resolution

anti-parallel Fc-knob (T366W) homodimer

Released:
DOI: 10.2210/pdb4nqu/pdb
PDB entry 4nqu coloured by chain, front view.
PDB entry 4nqu coloured by chain, side view.
PDB entry 4nqu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antiparallel conformation of knob and hole aglycosylated half-antibody homodimers is mediated by a CH2-CH3 hydrophobic interaction.
Elliott JM, Ultsch M, Lee J, Tong R, Takeda K, Spiess C, Eigenbrot C, Scheer JM
J Mol Biol 426 1947-57 (2014)
PMID: 24576605

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin heavy constant gamma 1 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01857 (Residues: 118-328; Coverage: 53%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3121
Unit cell:
a: 44.5Å b: 44.5Å c: 205.551Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.222 0.287
Expression system: Escherichia coli

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Citations

6 review citations

Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds.
Liu et al. (2017)
5 more