› 4nqx

X-ray diffraction
2Å resolution

Crystal Structure of HLA A*0101 in complex with NP44-S7N, an 9-mer influenza epitope

Released:
DOI: 10.2210/pdb4nqx/pdb
PDB entry 4nqx coloured by chain, front view.
PDB entry 4nqx coloured by chain, side view.
PDB entry 4nqx coloured by chain, top view.
Source organisms:
Primary publication:
Preexisting CD8+ T-cell immunity to the H7N9 influenza A virus varies across ethnicities.
Quiñones-Parra S, Grant E, Loh L, Nguyen TH, Campbell KA, Tong SY, Miller A, Doherty PC, Vijaykrishna D, Rossjohn J, Gras S, Kedzierska K
Proc Natl Acad Sci U S A 111 1049-54 (2014)
PMID: 24395804

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137804 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 284 amino acids
Theoretical weight: 32.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-308; Coverage: 83%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Nucleoprotein Chains: M, N, O, P, Q, R
Molecule details ›
Chains: M, N, O, P, Q, R
Length: 9 amino acids
Theoretical weight: 1.1 KDa
Source organism: H7N9 subtype
Expression system: Not provided
UniProt:
  • Canonical: Q07FI1 (Residues: 44-52; Coverage: 2%)
Gene name: NP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 264.61Å b: 81.411Å c: 140.29Å
α: 90° β: 121.82° γ: 90°
R-values:
R R work R free
0.27 0.27 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

38 review citations

Influenza.
Krammer et al. (2018)
37 more

9 mentions without citation

Preexisting CD8+ T-cell immunity to the H7N9 influenza A virus varies across ethnicities.
Quiñones-Parra et al. (2014)
8 more