› 4nr1

X-ray diffraction
2.68Å resolution

Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)allyl-GLY peptide

Released:
DOI: 10.2210/pdb4nr1/pdb
PDB entry 4nr1 coloured by chain, front view.
PDB entry 4nr1 coloured by chain, side view.
PDB entry 4nr1 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Scotti JS, McDonough MA, Schofield CJ

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164914 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:
CONSENSUS ANKYRIN REPEAT DOMAIN-(D)allyl glycine Chain: S
Molecule details ›
Chain: S
Length: 20 amino acids
Theoretical weight: 2.22 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand OGA 1 x OGA
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41212
Unit cell:
a: 86.37Å b: 86.37Å c: 148.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.21
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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