› 4nre

X-ray diffraction
2.63Å resolution

The structure of human 15-lipoxygenase-2 with a substrate mimic

Released:
DOI: 10.2210/pdb4nre/pdb
PDB entry 4nre coloured by chain, front view.
PDB entry 4nre coloured by chain, side view.
PDB entry 4nre coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human 15-lipoxygenase-2 with a substrate mimic.
Kobe MJ, Neau DB, Mitchell CE, Bartlett SG, Newcomer ME
J Biol Chem 289 8562-9 (2014)
PMID: 24497644

Function and Biology Details

Reaction catalysed: 
Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-127425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyunsaturated fatty acid lipoxygenase ALOX15B Chain: A
Molecule details ›
Chain: A
Length: 696 amino acids
Theoretical weight: 78.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15296 (Residues: 1-676; Coverage: 100%)
Gene name: ALOX15B
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand FE2 1 x FE2
Ligand C8E 2 x C8E
Ligand SO4 12 x SO4
Ligand CA 2 x CA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: R32
Unit cell:
a: 155.794Å b: 155.794Å c: 263.219Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.163 0.203
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Mammalian lipoxygenases and their biological relevance.
Kuhn et al. (2015)
6 more

42 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
41 more