› 4nrv

X-ray diffraction
2.6Å resolution

Crystal Structure of non-edited human NEIL1

Released:
DOI: 10.2210/pdb4nrv/pdb
PDB entry 4nrv coloured by chain, front view.
PDB entry 4nrv coloured by chain, side view.
PDB entry 4nrv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Genome and cancer single nucleotide polymorphisms of the human NEIL1 DNA glycosylase: activity, structure, and the effect of editing.
Prakash A, Carroll BL, Sweasy JB, Wallace SS, Doublié S
DNA Repair (Amst) 14 17-26 (2014)
PMID: 24382305

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease 8-like 1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96FI4 (Residues: 2-290; Coverage: 74%)
Gene name: NEIL1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R3
Unit cell:
a: 132.76Å b: 132.76Å c: 50.594Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.178 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Base excision repair: a critical player in many games.
Wallace SS. (2014)
5 more