› 4nuv

X-ray diffraction
2.6Å resolution

Heterotetramer structure of Region II from Plasmodium vivax Duffy Binding Protein (PvDBP) bound to the ectodomain of the Duffy Antigen Receptor for Chemokines (DARC)

Released:
DOI: 10.2210/pdb4nuv/pdb
PDB entry 4nuv coloured by chain, front view.
PDB entry 4nuv coloured by chain, side view.
PDB entry 4nuv coloured by chain, top view.
Source organisms:
Primary publication:
Red blood cell invasion by Plasmodium vivax: structural basis for DBP engagement of DARC.
Batchelor JD, Malpede BM, Omattage NS, DeKoster GT, Henzler-Wildman KA, Tolia NH
PLoS Pathog 10 e1003869 (2014)
PMID: 24415938

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149571 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Duffy receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 37.61 KDa
Source organism: Plasmodium vivax Sal-1
Expression system: Escherichia coli
UniProt:
  • Canonical: P22290 (Residues: 211-525; Coverage: 30%)
Gene name: PVDR
Sequence domains: Duffy binding domain
Structure domains:
Atypical chemokine receptor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 34 amino acids
Theoretical weight: 3.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16570 (Residues: 14-43; Coverage: 9%)
Gene names: ACKR1, DARC, FY, GPD

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P1
Unit cell:
a: 37.287Å b: 59.391Å c: 91.395Å
α: 103.14° β: 91.04° γ: 100.31°
R-values:
R R work R free
0.185 0.183 0.233
Expression system: Escherichia coli

Quick links

Citations

17 review citations

A guide to chemokines and their receptors.
Hughes et al. (2018)
16 more

18 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
17 more