› 4nwu

X-ray diffraction
1.6Å resolution

Crystal structure of APE1551, an anti-human NGF Fab with a nine amino acid insertion in CDR H1

Released:
DOI: 10.2210/pdb4nwu/pdb
PDB entry 4nwu coloured by chain, front view.
PDB entry 4nwu coloured by chain, side view.
PDB entry 4nwu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies.
Bowers PM, Verdino P, Wang Z, da Silva Correia J, Chhoa M, Macondray G, Do M, Neben TY, Horlick RA, Stanfield RL, Wilson IA, King DJ
J Biol Chem 289 33557-67 (2014)
PMID: 25320089

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134837 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin heavy constant gamma 1 Chain: H
Molecule details ›
Chain: H
Length: 242 amino acids
Theoretical weight: 25.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 1-105; Coverage: 26%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 24.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8TCD0 (Residues: 21-55, 56-239; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 127.053Å b: 66.256Å c: 63.884Å
α: 90° β: 108.54° γ: 90°
R-values:
R R work R free
0.168 0.166 0.198
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Monoclonal antibodies: technologies for early discovery and engineering.
Kennedy et al. (2018)
6 more

1 mention without citation

Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies.
Bowers et al. (2014)