› 4nzq

X-ray diffraction
2.81Å resolution

Crystal structure of Ca2+-free prothrombin deletion mutant residues 146-167

Released:
DOI: 10.2210/pdb4nzq/pdb
PDB entry 4nzq coloured by chain, front view.
PDB entry 4nzq coloured by chain, side view.
PDB entry 4nzq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The linker connecting the two kringles plays a key role in prothrombin activation.
Pozzi N, Chen Z, Pelc LA, Shropshire DB, Di Cera E
Proc Natl Acad Sci U S A 111 7630-5 (2014)
PMID: 24821807

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prothrombin Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 63.56 KDa
Source organism: Homo sapiens
Expression system: Cricetinae
UniProt:
  • Canonical: P00734 (Residues: 44-622; Coverage: 93%)
Gene name: F2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
1 modified residue:
Ligand CGU 10 x CGU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 88.754Å b: 171.739Å c: 141.252Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.279
Expression system: Cricetinae

Quick links

Citations

3 review citations

Plasminogen Activator Inhibitor Type-1 as a Regulator of Fibrosis.
Rabieian et al. (2018)
2 more

5 mentions without citation

The linker connecting the two kringles plays a key role in prothrombin activation.
Pozzi et al. (2014)
4 more