› 4o51

X-ray diffraction
2.2Å resolution

Crystal structure of the QAA variant of anti-hinge rabbit antibody 2095-2 in complex with IDES hinge peptide

Released:
DOI: 10.2210/pdb4o51/pdb
PDB entry 4o51 coloured by chain, front view.
PDB entry 4o51 coloured by chain, side view.
PDB entry 4o51 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and specificity of an antibody targeting a proteolytically cleaved IgG hinge.
Malia TJ, Teplyakov A, Brezski RJ, Luo J, Kinder M, Sweet RW, Almagro JC, Jordan RE, Gilliland GL
Proteins 82 1656-67 (2014)
PMID: 24638881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208630 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
QAA-2095-2 light chain Chains: A, C, E, L
Molecule details ›
Chains: A, C, E, L
Length: 216 amino acids
Theoretical weight: 23.19 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
QAA-2095-2 heavy chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 223 amino acids
Theoretical weight: 23.57 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 14 amino acids
Theoretical weight: 1.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01857 (Residues: 106-119; Coverage: 4%)
Gene name: IGHG1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 8 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 128.51Å b: 93.87Å c: 74.73Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.184 0.182 0.213
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

2 review citations

From rabbit antibody repertoires to rabbit monoclonal antibodies.
Weber et al. (2017)
1 more

1 mention without citation

Molecular Characterization of Seasonal Influenza A and B from Hospitalized Patients in Thailand in 2018-2019.
Boonnak et al. (2021)