› 4o58

X-ray diffraction
2.75Å resolution

Crystal structure of broadly neutralizing antibody F045-092 in complex with A/Victoria/3/1975 (H3N2) influenza hemagglutinin

Released:
DOI: 10.2210/pdb4o58/pdb
PDB entry 4o58 coloured by chain, front view.
PDB entry 4o58 coloured by chain, side view.
PDB entry 4o58 coloured by chain, top view.
Source organisms:
Primary publication:
Receptor mimicry by antibody F045-092 facilitates universal binding to the H3 subtype of influenza virus.
Lee PS, Ohshima N, Stanfield RL, Yu W, Iba Y, Okuno Y, Kurosawa Y, Wilson IA
Nat Commun 5 3614 (2014)
PMID: 24717798

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-208943 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin HA1 chain Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.45 KDa
Source organism: Influenza A virus (A/Victoria/3/1975(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03435 (Residues: 28-346; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.23 KDa
Source organism: Influenza A virus (A/Victoria/3/1975(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03435 (Residues: 347-522; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Fab F045-092 light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 22.63 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 25.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: S6C4S0 (Residues: 20-119, 120-128, 129-245; Coverage: 88%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 14 x SO4
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P321
Unit cell:
a: 99.101Å b: 99.101Å c: 336.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.224
Expression system: Trichoplusia ni

Quick links

Citations

33 review citations

Advances in the development of influenza virus vaccines.
Krammer et al. (2015)
32 more

26 mentions without citation

Antibody responses to viral infections: a structural perspective across three different enveloped viruses.
Murin et al. (2019)
25 more