› 4o62

X-ray diffraction
1.78Å resolution

CW-type zinc finger of ZCWPW2 in complex with the amino terminus of histone H3

Released:
DOI: 10.2210/pdb4o62/pdb
PDB entry 4o62 coloured by chain, front view.
PDB entry 4o62 coloured by chain, side view.
PDB entry 4o62 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Family-wide Characterization of Histone Binding Abilities of Human CW Domain-containing Proteins.
Liu Y, Tempel W, Zhang Q, Liang X, Loppnau P, Qin S, Min J
J Biol Chem 291 9000-13 (2016)
PMID: 26933034

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160860 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Zinc finger CW-type PWWP domain protein 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 59 amino acids
Theoretical weight: 7.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q504Y3 (Residues: 21-78; Coverage: 16%)
Gene name: ZCWPW2
Sequence domains: CW-type Zinc Finger
Structure domains: Herpes Virus-1
Histone H3.3 Chain: D
Molecule details ›
Chain: D
Length: 11 amino acids
Theoretical weight: 1.29 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 2-12; Coverage: 8%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand UNX 27 x UNX
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 127.095Å b: 127.095Å c: 63.046Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.186
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Structural insight into inhibitors of flavin adenine dinucleotide-dependent lysine demethylases.
Niwa et al. (2017)
5 more

3 mentions without citation

Family-wide Characterization of Histone Binding Abilities of Human CW Domain-containing Proteins.
Liu et al. (2016)
2 more