› 4o63

X-ray diffraction
1.93Å resolution

Co-enzyme Induced Conformational Changes in Bovine Eye Glyceraldehyde 3-Phosphate Dehydrogenase

Released:
DOI: 10.2210/pdb4o63/pdb
PDB entry 4o63 coloured by chain, front view.
PDB entry 4o63 coloured by chain, side view.
PDB entry 4o63 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
High-resolution crystal structures of the photoreceptor glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with three and four-bound NAD molecules.
Baker BY, Shi W, Wang B, Palczewski K
Protein Sci 23 1629-39 (2014)
PMID: 25176140

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 332 amino acids
Theoretical weight: 35.78 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P10096 (Residues: 2-333; Coverage: 100%)
Gene names: GAPD, GAPDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 3 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 79.752Å b: 126.205Å c: 83.228Å
α: 90° β: 118.13° γ: 90°
R-values:
R R work R free
0.19 0.188 0.234

Quick links

Citations

1 review citation

The sweet side of RNA regulation: glyceraldehyde-3-phosphate dehydrogenase as a noncanonical RNA-binding protein.
White et al. (2016)
7 other articles

2 mentions without citation

High-resolution crystal structures of the photoreceptor glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with three and four-bound NAD molecules.
Baker et al. (2014)
1 more