› 4o67

X-ray diffraction
2.44Å resolution

Human cyclic GMP-AMP synthase (cGAS) in complex with GAMP

Released:
DOI: 10.2210/pdb4o67/pdb
PDB entry 4o67 coloured by chain, front view.
PDB entry 4o67 coloured by chain, side view.
PDB entry 4o67 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cytosolic DNA sensor cGAS forms an oligomeric complex with DNA and undergoes switch-like conformational changes in the activation loop.
Zhang X, Wu J, Du F, Xu H, Sun L, Chen Z, Brautigam CA, Zhang X, Chen ZJ
Cell Rep 6 421-30 (2014)
PMID: 24462292

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 42.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 161-522; Coverage: 69%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1SY 2 x 1SY
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 47.822Å b: 118.542Å c: 124.025Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.2 0.281
Expression system: Escherichia coli

Quick links

Citations

124 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
123 more

6 mentions without citation

The cytosolic DNA sensor cGAS forms an oligomeric complex with DNA and undergoes switch-like conformational changes in the activation loop.
Zhang et al. (2014)
5 more