› 4o9r

X-ray diffraction
3.2Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein smoothened; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 52.4 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q99835 (Residues: 190-433, 441-555; Coverage: 47%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: SMO, SMOH, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CY8 1 x CY8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P21
Unit cell:
a: 40.5Å b: 157.3Å c: 52.4Å
α: 90° β: 97° γ: 90°
R-values:
R R work R free
0.234 0.232 0.278
Expression system: Spodoptera frugiperda

Quick links

Citations

55 review citations

Hedgehog Signaling: From Basic Biology to Cancer Therapy.
Wu et al. (2017)
54 more

35 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
34 more