› 4o9w

X-ray diffraction
1.69Å resolution

Crystal structure of polo-like kinase(PLK1)PBD in complex with phospho peptide

Released:
DOI: 10.2210/pdb4o9w/pdb
PDB entry 4o9w coloured by chain, front view.
PDB entry 4o9w coloured by chain, side view.
PDB entry 4o9w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The condensin component NCAPG2 regulates microtubule-kinetochore attachment through recruitment of Polo-like kinase 1 to kinetochores.
Kim JH, Shim J, Ji MJ, Jung Y, Bong SM, Jang YJ, Yoon EK, Lee SJ, Kim KG, Kim YH, Lee C, Lee BI, Kim KT
Nat Commun 5 4588 (2014)
PMID: 25109385

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156775 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 25.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 373-594; Coverage: 37%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
phospho peptide VAL-LEU-SER-TPO-LEU-NH2 Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 610 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 35.649Å b: 51.858Å c: 57.654Å
α: 90° β: 100.69° γ: 90°
R-values:
R R work R free
0.203 0.201 0.24
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Kinase and Phosphatase Cross-Talk at the Kinetochore.
Saurin AT. (2018)
3 more

1 mention without citation

Network Pharmacological Analysis through a Bioinformatics Approach of Novel NSC765600 and NSC765691 Compounds as Potential Inhibitors of CCND1/CDK4/PLK1/CD44 in Cancer Types.
Mokgautsi et al. (2021)