› 4oau

X-ray diffraction
2.6Å resolution

Complete human RNase L in complex with biological activators.

Released:
DOI: 10.2210/pdb4oau/pdb
PDB entry 4oau coloured by chain, front view.
PDB entry 4oau coloured by chain, side view.
PDB entry 4oau coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response.
Han Y, Donovan J, Rath S, Whitney G, Chitrakar A, Korennykh A
Science 343 1244-8 (2014)
PMID: 24578532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170255 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
2-5A-dependent ribonuclease Chain: C
Molecule details ›
Chain: C
Length: 699 amino acids
Theoretical weight: 79.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05823 (Residues: 21-719; Coverage: 94%)
Gene names: RNASEL, RNS4
Sequence domains:
Structure domains:
RNA (5'-R(P*A*AP*A)-2') Chain: A
Molecule details ›
Chain: A
Length: 3 nucleotides
Theoretical weight: 943 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Unit cell:
a: 60.13Å b: 116.6Å c: 162.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

32 review citations

The expanding regulatory mechanisms and cellular functions of circular RNAs.
Chen LL. (2020)
31 more

5 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
4 more