› 4ob5

X-ray diffraction
1.7Å resolution

Ontogeny of recognition specificity and functionality for the broadly neutralizing anti-HIV antibody 4E10

Released:
DOI: 10.2210/pdb4ob5/pdb
PDB entry 4ob5 coloured by chain, front view.
PDB entry 4ob5 coloured by chain, side view.
PDB entry 4ob5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ontogeny of recognition specificity and functionality for the broadly neutralizing anti-HIV antibody 4E10.
Finton KA, Friend D, Jaffe J, Gewe M, Holmes MA, Larman HB, Stuart A, Larimore K, Greenberg PD, Elledge SJ, Stamatatos L, Strong RK
PLoS Pathog 10 e1004403 (2014)
PMID: 25254371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208456 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4E10 antibody germline precursor 7 heavy chain Fv Chain: H
Molecule details ›
Chain: H
Length: 127 amino acids
Theoretical weight: 13.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin kappa variable 3-20 Chain: L
Molecule details ›
Chain: L
Length: 114 amino acids
Theoretical weight: 12.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01619 (Residues: 21-116; Coverage: 100%)
Gene name: IGKV3-20
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand TMO 1 x TMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 35.535Å b: 48.947Å c: 117.272Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Germline-targeting immunogens.
Stamatatos et al. (2017)
17 other articles

2 mentions without citation

Ontogeny of recognition specificity and functionality for the broadly neutralizing anti-HIV antibody 4E10.
Finton et al. (2014)
1 more