› 4okh

X-ray diffraction
2.45Å resolution

Crystal structure of calpain-3 penta-EF-hand domain

Released:
DOI: 10.2210/pdb4okh/pdb
PDB entry 4okh coloured by chain, front view.
PDB entry 4okh coloured by chain, side view.
PDB entry 4okh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of calpain-3 penta-EF-hand (PEF) domain - a homodimerized PEF family member with calcium bound at the fifth EF-hand.
Partha SK, Ravulapalli R, Allingham JS, Campbell RL, Davies PL
FEBS J 281 3138-49 (2014)
PMID: 24846670

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase activity
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calpain-3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 189 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20807 (Residues: 642-821; Coverage: 22%)
Gene names: CANP3, CANPL3, CAPN3, NCL1
Sequence domains: EF-hand domain
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 11 x CA
Ligand PEU 2 x PEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P41212
Unit cell:
a: 107Å b: 107Å c: 96.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.198 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Calpain-14 and its association with eosinophilic esophagitis.
Litosh et al. (2017)
2 more

4 mentions without citation

Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
Toral-Ojeda et al. (2016)
3 more