› 4ola

X-ray diffraction
2.3Å resolution

Crystal Structure of Human Argonaute2

Released:
DOI: 10.2210/pdb4ola/pdb
PDB entry 4ola coloured by chain, front view.
PDB entry 4ola coloured by chain, side view.
PDB entry 4ola coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human Argonaute2.
Schirle NT, MacRae IJ
Science 336 1037-40 (2012)
PMID: 22539551

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193866 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 859 amino acids
Theoretical weight: 97.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

2 bound ligands:
Ligand IPH 1 x IPH
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1, APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 63.157Å b: 107.669Å c: 68.522Å
α: 90° β: 107.1° γ: 90°
R-values:
R R work R free
0.214 0.212 0.253
Expression system: Spodoptera frugiperda

Quick links

Citations

105 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
104 more

18 mentions without citation

Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?
Nakanishi K. (2016)
17 more