› 4olb

X-ray diffraction
2.9Å resolution

Crystal Structure of Human Argonaute2 Bound to Tryptophan

Released:
DOI: 10.2210/pdb4olb/pdb
PDB entry 4olb coloured by chain, front view.
PDB entry 4olb coloured by chain, side view.
PDB entry 4olb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human Argonaute2.
Schirle NT, MacRae IJ
Science 336 1037-40 (2012)
PMID: 22539551

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193867 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 859 amino acids
Theoretical weight: 97.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 63.207Å b: 106.747Å c: 68.341Å
α: 90° β: 107.01° γ: 90°
R-values:
R R work R free
0.212 0.21 0.249
Expression system: Spodoptera frugiperda

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Citations

106 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
105 more

6 mentions without citation

From guide to target: molecular insights into eukaryotic RNA-interference machinery.
Ipsaro et al. (2015)
5 more