› 4oli

X-ray diffraction
2.8Å resolution

The pseudokinase/kinase protein from JAK-family member TYK2

Released:
DOI: 10.2210/pdb4oli/pdb
PDB entry 4oli coloured by chain, front view.
PDB entry 4oli coloured by chain, side view.
PDB entry 4oli coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the pseudokinase-kinase domains from protein kinase TYK2 reveals a mechanism for Janus kinase (JAK) autoinhibition.
Lupardus PJ, Ultsch M, Wallweber H, Bir Kohli P, Johnson AR, Eigenbrot C
Proc Natl Acad Sci U S A 111 8025-30 (2014)
PMID: 24843152

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-receptor tyrosine-protein kinase TYK2 Chain: A
Molecule details ›
Chain: A
Length: 645 amino acids
Theoretical weight: 73.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29597 (Residues: 566-1187; Coverage: 52%)
Gene name: TYK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 2TT 2 x 2TT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I4
Unit cell:
a: 111.015Å b: 111.015Å c: 123.955Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.201 0.256
Expression system: Spodoptera frugiperda

Quick links

Citations

42 review citations

Janus kinase (JAK) inhibitors in the treatment of inflammatory and neoplastic diseases.
Roskoski R. (2016)
41 more

23 mentions without citation

The molecular details of cytokine signaling via the JAK/STAT pathway.
Morris et al. (2018)
22 more