› 4omt

X-ray diffraction
6Å resolution

Crystal structure of human muscle phosphofructokinase (dissociated homodimer)

Released:
DOI: 10.2210/pdb4omt/pdb
PDB entry 4omt coloured by chain, front view.
PDB entry 4omt coloured by chain, side view.
PDB entry 4omt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallization and preliminary crystallographic analysis of human muscle phosphofructokinase, the main regulator of glycolysis.
Kloos M, Brüser A, Kirchberger J, Schöneberg T, Sträter N
Acta Crystallogr F Struct Biol Commun 70 578-82 (2014)
PMID: 24817713

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase, muscle type Chain: A
Molecule details ›
Chain: A
Length: 780 amino acids
Theoretical weight: 85.29 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08237 (Residues: 1-780; Coverage: 100%)
Gene names: PFKM, PFKX
Sequence domains: Phosphofructokinase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6222
Unit cell:
a: 229.7Å b: 229.7Å c: 133Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.238 0.274
Expression system: Saccharomyces cerevisiae

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Citations

1 review citation

Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation.
Park et al. (2019)
1 other article

4 mentions without citation

The sarcomeric M-region: a molecular command center for diverse cellular processes.
Hu et al. (2015)
3 more