› 4or2

X-ray diffraction
2.8Å resolution

Human class C G protein-coupled metabotropic glutamate receptor 1 in complex with a negative allosteric modulator

Released:
DOI: 10.2210/pdb4or2/pdb
PDB entry 4or2 coloured by chain, front view.
PDB entry 4or2 coloured by chain, side view.
PDB entry 4or2 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a class C GPCR metabotropic glutamate receptor 1 bound to an allosteric modulator.
Wu H, Wang C, Gregory KJ, Han GW, Cho HP, Xia Y, Niswender CM, Katritch V, Meiler J, Cherezov V, Conn PJ, Stevens RC
Science 344 58-64 (2014)
PMID: 24603153

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metabotropic glutamate receptor 1; Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.25 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: Q13255 (Residues: 581-860; Coverage: 24%)
Gene names: GPRC1A, GRM1, MGLUR1, cybC
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand FM9 2 x FM9
Ligand CLR 6 x CLR
Ligand OLA 1 x OLA
Ligand OLC 3 x OLC
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 67.361Å b: 86.552Å c: 168.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.268
Expression system: Spodoptera frugiperda

Quick links

Citations

72 review citations

Glutamatergic Signaling in the Central Nervous System: Ionotropic and Metabotropic Receptors in Concert.
Reiner et al. (2018)
71 more

96 mentions without citation

Generic GPCR residue numbers - aligning topology maps while minding the gaps.
Isberg et al. (2015)
95 more