› 4os5

X-ray diffraction
2.26Å resolution

Crystal structure of urokinase-type plasminogen activator (uPA) complexed with bicyclic peptide UK603 (bicyclic 2)

Released:
DOI: 10.2210/pdb4os5/pdb
PDB entry 4os5 coloured by chain, front view.
PDB entry 4os5 coloured by chain, side view.
PDB entry 4os5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides.
Chen S, Gopalakrishnan R, Schaer T, Marger F, Hovius R, Bertrand D, Pojer F, Heinis C
Nat Chem 6 1009-16 (2014)
PMID: 25343607

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133272 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00749 (Residues: 179-423; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
bicyclic peptide UK603 (bicyclic 2) Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.55 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 52.393Å b: 53.934Å c: 82.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.2
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

10 review citations

Structure-Based Design of Inhibitors of Protein-Protein Interactions: Mimicking Peptide Binding Epitopes.
Pelay-Gimeno et al. (2015)
9 more