› 4ouc

X-ray diffraction
1.9Å resolution

Structure of human haspin in complex with histone H3 substrate

Released:
DOI: 10.2210/pdb4ouc/pdb
PDB entry 4ouc coloured by chain, front view.
PDB entry 4ouc coloured by chain, side view.
PDB entry 4ouc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modulation of the chromatin phosphoproteome by the Haspin protein kinase.
Maiolica A, de Medina-Redondo M, Schoof EM, Chaikuad A, Villa F, Gatti M, Jeganathan S, Lou HJ, Novy K, Hauri S, Toprak UH, Herzog F, Meraldi P, Penengo L, Turk BE, Knapp S, Linding R, Aebersold R
Mol Cell Proteomics 13 1724-40 (2014)
PMID: 24732914

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180739 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TF76 (Residues: 465-798; Coverage: 42%)
Gene names: GSG2, HASPIN
Sequence domains: Haspin like kinase domain
Structure domains:
Histone H3.2 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71DI3 (Residues: 2-13; Coverage: 9%)
Gene names: H3C13, H3C14, H3C15, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand EDO 6 x EDO
Ligand 5ID 1 x 5ID
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 50.54Å b: 79.13Å c: 100.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.211
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Post-Translational Modifications of H2A Histone Variants and Their Role in Cancer.
Corujo et al. (2018)
4 more

6 mentions without citation

Homing in: Mechanisms of Substrate Targeting by Protein Kinases.
Miller et al. (2018)
5 more