› 4ovn

X-ray diffraction
2.8Å resolution

Voltage-gated Sodium Channel 1.5 (Nav1.5) C-terminal domain in complex with Calmodulin poised for activation

Released:
DOI: 10.2210/pdb4ovn/pdb
PDB entry 4ovn coloured by chain, front view.
PDB entry 4ovn coloured by chain, side view.
PDB entry 4ovn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of the NaV1.5 cytoplasmic domain by calmodulin.
Gabelli SB, Boto A, Kuhns VH, Bianchet MA, Farinelli F, Aripirala S, Yoder J, Jakoncic J, Tomaselli GF, Amzel LM
Nat Commun 5 5126 (2014)
PMID: 25370050

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143949 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 149 amino acids
Theoretical weight: 17.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Sodium channel protein type 5 subunit alpha Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 157 amino acids
Theoretical weight: 18.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14524 (Residues: 1773-1929; Coverage: 8%)
Gene name: SCN5A
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 18 x MG
Ligand SO4 5 x SO4
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 90 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 106.128Å b: 99.006Å c: 109.281Å
α: 90° β: 106.11° γ: 90°
R-values:
R R work R free
0.215 0.211 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

8 review citations

Ion channel macromolecular complexes in cardiomyocytes: roles in sudden cardiac death.
Abriel et al. (2015)
7 more

20 mentions without citation

Current view on regulation of voltage-gated sodium channels by calcium and auxiliary proteins.
Pitt et al. (2016)
19 more